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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
05/12/2023 |
Actualizado : |
24/07/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
Autor : |
MORALES-PIÑEYRUA, J.; BOBADILLA, P.E.; ALCANTARA, I.; DE TORRES, E. |
Afiliación : |
JESSICA TATIANA MORALES PIÑEYRUA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ERNESTO BOBADILLA, Universidad de la República, Facultad de Veterinaria, Unidad de Bioestadística Departamento de Salud Pública Veterinaria, Montevideo, Uruguay; IGNACIO ALCANTARA, Universidad de la República, Facultad de Veterinaria, Unidad de Bioestadística Departamento de Salud Pública Veterinaria, Montevideo, Uruguay; ELENA DE TORRES, Universidad de la República, Facultad de Veterinaria, Campo Experimental N.°2, San José, Uruguay. |
Título : |
Relationship between strains of Holstein cows, feeding strategies and udder health. [Relación entre el biotipo de vacas Holando, la estrategia de alimentación y la salud de ubre]. [Relação entre o biótipo de vacas Holando, a estratégia de alimentação e a saúde do úbere]. |
Complemento del título : |
Animal production and pastures. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Agrociencia Uruguay, 2023, Vol.27, e1150. https://doi.org/10.31285/AGRO.27.1150 -- OPEN ACCESS. |
ISSN : |
2730-5066 |
DOI : |
10.31285/AGRO.27.1150 |
Idioma : |
Inglés |
Notas : |
Article history: Received 29 Nov 2022; Accepted 15 Jun 2023; Published 10 Jul 2023. -- Editor: Laura Astigarraga, Universidad de la República, Facultad de Agronomía, Montevideo, Uruguay. -- Correspondence: Jéssica Tatiana Morales-Piñeyrúa, email: jmorales@inia.org.uy -- License: This work is licensed under a Creative Commons Attribution 4.0 International License. (https://creativecommons.org/licenses/by/4.0/ ) |
Contenido : |
ABSTRACT.- Mastitis frequency could be affected by animal and environmental conditions such as dairy cow genetics and feeding strategies. Thus, the objective of this study was to analyze the probability of clinical mastitis and somatic cell count (SCC) for New Zealand (NZ) and North American (NA) Holstein-Friesian cows maintained on two different feeding strategies (pasture or mixed). A total of 120 cows from the experimental dairy farm of the National Agricultural and Livestock Re-search Institute (INIA, by its Spanish acronym) (Colonia, Uruguay) were grouped into four groups derived from the combination of two feeding strategies: Grass Maximum or Grass Fixed, and two Holstein strains: NZ or NA (n=30). Clinical mastitis and SCC were evaluated monthly during a whole lactation period. There was an interaction between the Holstein strains and parity for clinical mastitis (P=0.04). The NA primiparous cows were the least likely to suffer clinical mastitis (OR: 0.003), while NA multiparous cows were the most likely to show clinical mastitis (OR: 0.12). The NZ cows reported intermediate values of OR (primiparous: 0.082, multiparous: 0.066). Feeding strategies did not affect clinical mastitis or SCC. Similar SCC was found for NZ and NA strains. In conclusion, regardless of the feeding strategy, the probability of clinical mastitis differed by the genetic origin of the Holstein cows. However, this difference was influenced by parity. The SCC was not influenced by the Holstein strain or the feeding strategy. ---------- RESUMEN.- Factores relacionados con el animal y el ambiente pueden afectar la frecuencia de mastitis, siendo la genética y la estrategia de alimentación algunos de dichos factores. El objetivo de este estudio fue analizar la frecuencia de mastitis clínica y los recuentos de células somáticas (RCS) de vacas Holando de dos biotipos, Norteamericano (NA) y Neozelandés (NZ), bajo dos estrategias de alimentación (pastura o mixto). En la Unidad Experimental de Lechería de INIA La Estanzuela (Colonia, Uruguay) fueron agrupadas 120 vacas en 4 tratamientos según la combinación de biotipo y estrategia de alimentación (n=30). La cantidad de vacas con mastitis clínica y los RCS fueron registrados mensualmente durante una lactación. Hubo una interacción entre el biotipo y la paridad de las vacas (primíparas y multíparas) (P=0,04) para la frecuencia de mastitis. Vacas primíparas NA presentaron menor probabilidad de presentar mastitis clínica (ODR: 0,003). Por el contrario, las vacas multíparas NA fueron las que presentaron mayor probabilidad de experimentar mastitis clínica (ODR: 0,12), mientras que las vacas de origen NZ mostraron valores intermedios (ODR primíparas: 0,082; ODR multíparas: 0,066). La estrategia de alimentación no afectó la frecuencia de mastitis ni los RCS. Tampoco el biotipo afectó el RCS. En conclusión, sin importar la estrategia de alimentación, la frecuencia de mastitis clínica fue diferente según el biotipo, pero estas diferencias fueron influenciadas por el número de partos de los animales. Los RCS no fueron afectados por la estrategia de alimentación o el biotipo animal. ---------- RESUMO.- A genética e os sistemas de alimentação são fatores que afetam a incidência de mastite. Portanto, o objetivo deste estudo foi analisar a incidência de mastite clínica e a contagem de células somáticas (CCS) de vacas holandesas de dois biótipos, norte-americana (NA) e neozelandês (NZ), sob dois sistemas de alimentação (pastejo fixo ou máximo). Na Unidade Experimental do INIA La Estanzuela (Colonia, Uruguay) 120 vacas foram distribuídas em 4 tratamentos de acordo com a combinação de biótipo e alimentação (n = 30). O número de vacas com mastite clínica e a CSS foram registrados mensalmente durante uma lactação. Houve interação entre o biótipo e a paridade das vacas (P = 0,04) na frequência de mastite. Vacas primíparas NA apresentaram menor probabilidade de apresentarem mastite clínica (ODR: 0,003). Em contraste, as vacas multíparas NA tiveram a maior probabilidade de experimentar mastite clínica (ODR: 0,12), sendo as vacas de origem NZ os animais com valores intermédios (ODR primíparas: 0,082; ODR multíparas: 0,066). O sistema de alimentação não afetou a frequência de mastite ou CCS. O biótipo também não afetou a CCS. Em conclusão, independente do sistema de alimentação, a frequência de mastite foi diferente de acordo com o biótipo, mas essas diferenças foram influenciadas pela paridade dos animais. A CCS não foi afetada pelo sistema de alimentação ou biótipo animal. @Copyright (c) 2023 Agrociencia Uruguay. MenosABSTRACT.- Mastitis frequency could be affected by animal and environmental conditions such as dairy cow genetics and feeding strategies. Thus, the objective of this study was to analyze the probability of clinical mastitis and somatic cell count (SCC) for New Zealand (NZ) and North American (NA) Holstein-Friesian cows maintained on two different feeding strategies (pasture or mixed). A total of 120 cows from the experimental dairy farm of the National Agricultural and Livestock Re-search Institute (INIA, by its Spanish acronym) (Colonia, Uruguay) were grouped into four groups derived from the combination of two feeding strategies: Grass Maximum or Grass Fixed, and two Holstein strains: NZ or NA (n=30). Clinical mastitis and SCC were evaluated monthly during a whole lactation period. There was an interaction between the Holstein strains and parity for clinical mastitis (P=0.04). The NA primiparous cows were the least likely to suffer clinical mastitis (OR: 0.003), while NA multiparous cows were the most likely to show clinical mastitis (OR: 0.12). The NZ cows reported intermediate values of OR (primiparous: 0.082, multiparous: 0.066). Feeding strategies did not affect clinical mastitis or SCC. Similar SCC was found for NZ and NA strains. In conclusion, regardless of the feeding strategy, the probability of clinical mastitis differed by the genetic origin of the Holstein cows. However, this difference was influenced by parity. The SCC was not influenced by the Holstein strain... Presentar Todo |
Palabras claves : |
Calidad de leche; Conformação da úbere; Conformación de ubre; Grazed pasture; Milk quality; Pastagem; Pastoreo; Qualidade da leite; SISTEMA LECHERO - INIA; Udder conformation. |
Asunto categoría : |
L02 Alimentación animal |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17299/1/2730-5066-1150.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/17422/1/AGRO.27.1150.pdf
|
Marc : |
LEADER 06231naa a2200313 a 4500 001 1064379 005 2023-07-24 008 2023 bl uuuu u00u1 u #d 022 $a2730-5066 024 7 $a10.31285/AGRO.27.1150$2DOI 100 1 $aMORALES-PIÑEYRUA, J. 245 $aRelationship between strains of Holstein cows, feeding strategies and udder health. [Relación entre el biotipo de vacas Holando, la estrategia de alimentación y la salud de ubre]. [Relação entre o biótipo de vacas Holando, a estratégia de alimentação e a saúde do úbere].$h[electronic resource] 260 $c2023 500 $aArticle history: Received 29 Nov 2022; Accepted 15 Jun 2023; Published 10 Jul 2023. -- Editor: Laura Astigarraga, Universidad de la República, Facultad de Agronomía, Montevideo, Uruguay. -- Correspondence: Jéssica Tatiana Morales-Piñeyrúa, email: jmorales@inia.org.uy -- License: This work is licensed under a Creative Commons Attribution 4.0 International License. (https://creativecommons.org/licenses/by/4.0/ ) 520 $aABSTRACT.- Mastitis frequency could be affected by animal and environmental conditions such as dairy cow genetics and feeding strategies. Thus, the objective of this study was to analyze the probability of clinical mastitis and somatic cell count (SCC) for New Zealand (NZ) and North American (NA) Holstein-Friesian cows maintained on two different feeding strategies (pasture or mixed). A total of 120 cows from the experimental dairy farm of the National Agricultural and Livestock Re-search Institute (INIA, by its Spanish acronym) (Colonia, Uruguay) were grouped into four groups derived from the combination of two feeding strategies: Grass Maximum or Grass Fixed, and two Holstein strains: NZ or NA (n=30). Clinical mastitis and SCC were evaluated monthly during a whole lactation period. There was an interaction between the Holstein strains and parity for clinical mastitis (P=0.04). The NA primiparous cows were the least likely to suffer clinical mastitis (OR: 0.003), while NA multiparous cows were the most likely to show clinical mastitis (OR: 0.12). The NZ cows reported intermediate values of OR (primiparous: 0.082, multiparous: 0.066). Feeding strategies did not affect clinical mastitis or SCC. Similar SCC was found for NZ and NA strains. In conclusion, regardless of the feeding strategy, the probability of clinical mastitis differed by the genetic origin of the Holstein cows. However, this difference was influenced by parity. The SCC was not influenced by the Holstein strain or the feeding strategy. ---------- RESUMEN.- Factores relacionados con el animal y el ambiente pueden afectar la frecuencia de mastitis, siendo la genética y la estrategia de alimentación algunos de dichos factores. El objetivo de este estudio fue analizar la frecuencia de mastitis clínica y los recuentos de células somáticas (RCS) de vacas Holando de dos biotipos, Norteamericano (NA) y Neozelandés (NZ), bajo dos estrategias de alimentación (pastura o mixto). En la Unidad Experimental de Lechería de INIA La Estanzuela (Colonia, Uruguay) fueron agrupadas 120 vacas en 4 tratamientos según la combinación de biotipo y estrategia de alimentación (n=30). La cantidad de vacas con mastitis clínica y los RCS fueron registrados mensualmente durante una lactación. Hubo una interacción entre el biotipo y la paridad de las vacas (primíparas y multíparas) (P=0,04) para la frecuencia de mastitis. Vacas primíparas NA presentaron menor probabilidad de presentar mastitis clínica (ODR: 0,003). Por el contrario, las vacas multíparas NA fueron las que presentaron mayor probabilidad de experimentar mastitis clínica (ODR: 0,12), mientras que las vacas de origen NZ mostraron valores intermedios (ODR primíparas: 0,082; ODR multíparas: 0,066). La estrategia de alimentación no afectó la frecuencia de mastitis ni los RCS. Tampoco el biotipo afectó el RCS. En conclusión, sin importar la estrategia de alimentación, la frecuencia de mastitis clínica fue diferente según el biotipo, pero estas diferencias fueron influenciadas por el número de partos de los animales. Los RCS no fueron afectados por la estrategia de alimentación o el biotipo animal. ---------- RESUMO.- A genética e os sistemas de alimentação são fatores que afetam a incidência de mastite. Portanto, o objetivo deste estudo foi analisar a incidência de mastite clínica e a contagem de células somáticas (CCS) de vacas holandesas de dois biótipos, norte-americana (NA) e neozelandês (NZ), sob dois sistemas de alimentação (pastejo fixo ou máximo). Na Unidade Experimental do INIA La Estanzuela (Colonia, Uruguay) 120 vacas foram distribuídas em 4 tratamentos de acordo com a combinação de biótipo e alimentação (n = 30). O número de vacas com mastite clínica e a CSS foram registrados mensalmente durante uma lactação. Houve interação entre o biótipo e a paridade das vacas (P = 0,04) na frequência de mastite. Vacas primíparas NA apresentaram menor probabilidade de apresentarem mastite clínica (ODR: 0,003). Em contraste, as vacas multíparas NA tiveram a maior probabilidade de experimentar mastite clínica (ODR: 0,12), sendo as vacas de origem NZ os animais com valores intermédios (ODR primíparas: 0,082; ODR multíparas: 0,066). O sistema de alimentação não afetou a frequência de mastite ou CCS. O biótipo também não afetou a CCS. Em conclusão, independente do sistema de alimentação, a frequência de mastite foi diferente de acordo com o biótipo, mas essas diferenças foram influenciadas pela paridade dos animais. A CCS não foi afetada pelo sistema de alimentação ou biótipo animal. @Copyright (c) 2023 Agrociencia Uruguay. 653 $aCalidad de leche 653 $aConformação da úbere 653 $aConformación de ubre 653 $aGrazed pasture 653 $aMilk quality 653 $aPastagem 653 $aPastoreo 653 $aQualidade da leite 653 $aSISTEMA LECHERO - INIA 653 $aUdder conformation 700 1 $aBOBADILLA, P.E. 700 1 $aALCANTARA, I. 700 1 $aDE TORRES, E. 773 $tAgrociencia Uruguay, 2023, Vol.27, e1150. https://doi.org/10.31285/AGRO.27.1150 -- OPEN ACCESS.
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
07/07/2021 |
Actualizado : |
27/06/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
MANISSE, N.; CABRERA, A.; HERNÁN, J.; COSSE, M.; GIANNITTI, F.; FRANCIA, M.E.; GONZÁLEZ, T.; IRIARTE, A.; RIET-CORREA, F.; ROBELLO, C.; GONZÁLEZ, S. |
Afiliación : |
NATALIA MANISSE, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; ANDRÉS CABRERA, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; JUAN HERNÁN, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; MARIANA COSSE, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA E. FRANCIA, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; TELMA GONZÁLEZ, Departamento de Parasitología y Micología, Facultad de Medicina, Instituto de Higiene, Universidad de La República, Avenida Dr. Alfredo Navarro 3051, 11600, Montevideo, Uruguay.; ANDRÉS IRIARTE, Laboratorio de Biología Computacional, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Universidad de La República Facultad de Medicina, Avenida Dr. Alfredo Navarro 3051, 11600, Montevideo, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Programa de Pós-graduação em Ciência Animal nos Trópicos, Universidade Federal da Bahia, Salvador, Bahia, Brazil.; CARLOS ROBELLO, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; SUSANA GONZÁLEZ, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay. |
Título : |
Molecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes: a contribution to their molecular epidemiology. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Molecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w |
DOI : |
10.1007/s11033-021-06492-w |
Idioma : |
Inglés |
Notas : |
Article history: Received: 24 November 2020 / Accepted: 11 June 2021. This study obtained fnancial support from Programa de
Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación (FSSA_X_2014_1_106026). |
Contenido : |
Abstract:
Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as defnitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identifcation of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox=46, crab-eating fox=55) and feces (pampas fox=84, crab-eating fox=2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplifcation of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel realtime PCR TaqMan? probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the frst report of N. caninum DNA in pampas fox feces (n=1), thus it was also detected from pampas fox tissues (n=1). Meanwhile, T. gondiiwas found in tissues of pampas (n=1) and crab-eating (n=1) foxes and H. trifttae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife. |
Palabras claves : |
Hammondia spp; NEOSPOROSIS; Neotropical canids; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; Real-time PCR; TOXOPLASMOSIS. |
Asunto categoría : |
E16 Enfermedades de los animales |
Marc : |
LEADER 02795naa a2200349 a 4500 001 1062284 005 2022-06-27 008 2021 bl uuuu u00u1 u #d 024 7 $a10.1007/s11033-021-06492-w$2DOI 100 1 $aMANISSE, N. 245 $aMolecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes$ba contribution to their molecular epidemiology.$h[electronic resource] 260 $c2021 500 $aArticle history: Received: 24 November 2020 / Accepted: 11 June 2021. This study obtained fnancial support from Programa de Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación (FSSA_X_2014_1_106026). 520 $aAbstract: Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as defnitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identifcation of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox=46, crab-eating fox=55) and feces (pampas fox=84, crab-eating fox=2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplifcation of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel realtime PCR TaqMan? probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the frst report of N. caninum DNA in pampas fox feces (n=1), thus it was also detected from pampas fox tissues (n=1). Meanwhile, T. gondiiwas found in tissues of pampas (n=1) and crab-eating (n=1) foxes and H. trifttae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife. 653 $aHammondia spp 653 $aNEOSPOROSIS 653 $aNeotropical canids 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aReal-time PCR 653 $aTOXOPLASMOSIS 700 1 $aCABRERA, A. 700 1 $aHERNÁN, J. 700 1 $aCOSSE, M. 700 1 $aGIANNITTI, F. 700 1 $aFRANCIA, M.E. 700 1 $aGONZÁLEZ, T. 700 1 $aIRIARTE, A. 700 1 $aRIET-CORREA, F. 700 1 $aROBELLO, C. 700 1 $aGONZÁLEZ, S. 773 $tMolecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w
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